CRE BAG 2 (ATCC® CRL-1858)

Organism: Mus musculus, mouse  /  Disease: Leukemia

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Leukemia
Strain NIH/Swiss
Applications
produces protein beta-galactosidase beta-D-galactosidase
Derivation
The CRE BAG 2 cell line has never produced a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
Genes Expressed
a beta galactosidase transducing vector (BAG),beta-galactosidase(beta-D-galactosidase) [lacZ],beta-galactosidase
Cellular Products
a beta galactosidase transducing vector (BAG)
Comments
The BAG construct has been used to transfect psiCRE cells in ATCC CRL-1858 and to transfect psi2 cells in ATCC CRL-9560.
The CRE BAG 2 cell line has never produced a replication competent helper virus.
This line produces a vector (BAG) that can infect mouse and rat cells and transduce beta-galactosidase activity.
The line is similar to the psi 2 BAG alpha line (see ATCC CRL-9560) but has never been known to produce a replication competent helper virus.
The cells will package vectors derived from murine leukemia viruses.
The vector has been used to infect a variety of neural cell types and to express beta-galactosidase and resistance to G418.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; bovine calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin - 0.02% EDTA and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). Add fresh medium, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor CL Cepko
References

Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292

Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789

Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107

Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989.

Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Price J, et al. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84: 156-160, 1987. PubMed: 3099292

Turner DL, Cepko CL. A common progenitor for neurons and glia persists in rat retina late in development. Nature 328: 131-136, 1987. PubMed: 3600789

Danos O, Mulligan RC. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464, 1988. PubMed: 3413107

Cepko CLVirus propagation and Xgal proceduresIn: Cepko CLNeuromethodsClifton, NJHumana Press1989.

Le Doux JM, et al. Proteoglycans secreted by packaging cell lines inhibit retrovirus infection. J. Virol. 70: 6468-6473, 1996. PubMed: 8709284