RD (ATCC® CCL-136)

Organism: Homo sapiens, human  /  Tissue: muscle  /  Disease: rhabdomyosarcoma

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Organism Homo sapiens, human
Tissue muscle
Product Format frozen
Morphology spindle cells and large multinucleated cells
Culture Properties adherent
Biosafety Level 1
Disease rhabdomyosarcoma
Age 7 years
Gender female
Ethnicity Caucasian
Applications
This cell line can be used for the detection of viruses.
Storage Conditions liquid nitrogen vapor phase
Karyotype unstable within a hyperdiploid bimodal stemline number of 49 and 50. Twenty-two cells had chromosome associations, 15 cells microchromosomes, 2 cells had fragments, 2 cells with breaks, 2 cells with achromatic gaps and 1 cell with a secondary constriction Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images
Clinical Data
7 years
Caucasian
female
Genes Expressed myoglobin; myosin ATPase
Virus Susceptibility Human poliovirus 1
Vesicular stomatitis, Glasgow (Indiana)
Vesicular stomatitis, Orsay (Indiana)
Herpes simplex virus
Vaccinia virus
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,11
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 9
vWA: 18
Isoenzymes
G6PD, B
Name of Depositor RM McAllister
References

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949

Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733

Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385

Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236

Guerin E, et al. Stromelysin-3 induction and interstitial collagenase repression by retinoic acid. J. Biol. Chem. 272: 11088-11095, 1997. PubMed: 9111003

Jeffers M, et al. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol. Cell. Biol. 17: 799-808, 1997. PubMed: 9001234

Massuda ES, et al. Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proc. Natl. Acad. Sci. USA 94: 14701-14706, 1997. PubMed: 9405676

Tiger CF, et al. Presence of laminin alpha5 chain and lack laminin alpha1 chain during human muscle development and in muscular dystrophies. J. Biol. Chem. 272: 28590-28595, 1997. PubMed: 9353324

Sabbioni S, et al. GOK: a gene at 11p15 involved in rhabdomysarcoma and rhabdoid tumor development. Cancer Res. 57: 4493-4497, 1997. PubMed: 9377559

Xiao GH, et al. The Tuberous Sclerosis 2Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis. J. Biol. Chem. 272: 6097-6100, 1997. PubMed: 9045618

Cross References

Nucleotide (GenBank) : U09279 Human type XIX collagen (COL19A1) mRNA, partial cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.

McAllister RM, et al. Cultivation in vitro of cells derived from a human rhabdomyosarcoma. Cancer 24: 520-526, 1969. PubMed: 4241949

Chen TR, et al. Cell identity resolved. Nature 340: 106, 1989. PubMed: 2739733

Stratton MR, et al. Misidentified cell. Nature 337: 311-312, 1989. PubMed: 2911385

Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033

Hahon N, Cooke KO. Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. J. Virol. 1: 326, 1967. PubMed: 4918236

Guerin E, et al. Stromelysin-3 induction and interstitial collagenase repression by retinoic acid. J. Biol. Chem. 272: 11088-11095, 1997. PubMed: 9111003

Jeffers M, et al. Degradation of the Met tyrosine kinase receptor by the ubiquitin-proteasome pathway. Mol. Cell. Biol. 17: 799-808, 1997. PubMed: 9001234

Massuda ES, et al. Regulated expression of the diphtheria toxin A chain by a tumor-specific chimeric transcription factor results in selective toxicity for alveolar rhabdomyosarcoma cells. Proc. Natl. Acad. Sci. USA 94: 14701-14706, 1997. PubMed: 9405676

Tiger CF, et al. Presence of laminin alpha5 chain and lack laminin alpha1 chain during human muscle development and in muscular dystrophies. J. Biol. Chem. 272: 28590-28595, 1997. PubMed: 9353324

Sabbioni S, et al. GOK: a gene at 11p15 involved in rhabdomysarcoma and rhabdoid tumor development. Cancer Res. 57: 4493-4497, 1997. PubMed: 9377559

Xiao GH, et al. The Tuberous Sclerosis 2Gene Product, Tuberin, Functions as a Rab5 GTPase Activating Protein (GAP) in Modulating Endocytosis. J. Biol. Chem. 272: 6097-6100, 1997. PubMed: 9045618