IMR-32 (ATCC® CCL-127™)

Organism: Homo sapiens, human  /  Tissue: brain
Derived from Metastatic site: abdominal mass  /  Cell Type: neuroblast

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Organism Homo sapiens, human
Tissue brain
Derived from Metastatic site: abdominal mass
Cell Type neuroblast
Product Format frozen
Morphology fibroblast; neuroblast
Culture Properties adherent
Biosafety Level 1
Disease neuroblastoma
Age 13 months
Gender male
Ethnicity Caucasian
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype Stable male karyotype with stemline number of 49. Two large marker chromosomes with submedian centromeres. A deletion in one number 1 chromosome: One number 16 chromosome missing; two extra chromosomes in C group. Sublines with 50 and 48 chromosomes differ from those with 49 chromosomes by having an extra or missing C group chromosome respectively.
Images
Derivation
The IMR-32 cell line was established by W.W. Nichols, J. Lee and S. Dwight in April, 1967 from an abdominal mass occurring in a 13-month-old Caucasian male.
Clinical Data
13 months
Caucasian
male
Virus Resistance
echovirus 11
Comments
The tumor was diagnosed as a neuroblastoma with rare areas of organoid differentiation.

Two cell types are present. Predominant is a small neuroblast-like cell.The other is a large hyaline fibroblast.

ATCC CCL-127 cells may pile up and grow in patches. 

ATCC CCL-127 cells may not become 100% confluent.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Maintain cultures at a cell concentration between 4 x 104 and 4 x 105 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9
D16S539: 8
D5S818: 11,12
D7S820: 9,10
THO1: 7,9.3
TPOX: 11
vWA: 15
Isoenzymes
G6PD, B
Population Doubling Time approximately 20 hrs.
Name of Depositor WW Nichols
Passage History
The cell line was submitted to the American Type Culture Collection in the 36th passage. It has been demonstrated that the cells can be propagated successfully beyond the 80th serial subculture.
Year of Origin April, 1967
References

Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cell Micrograph
References

Tumilowicz JJ, et al. Definition of a continuous human cell line derived from neuroblastoma. Cancer Res. 30: 2110-2118, 1970. PubMed: 5459762

Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024

Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614