F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (ATCC® 30-2004)

Formulation Formulation
Product Format 500 mL
Storage Conditions Store medium at 2°C to 8°C in the dark when not in use.
Applications
NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air.
Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Comments
Modified to contain 2 mM L-glutamine and 1500 mg/L sodium bicarbonate.NOTE: This reduced level of sodium bicarbonate (NaHCO3, 1.5 g/L) is intended for use in 5% CO2 in air. Additional sodium bicarbonate may be required for use in incubators containing higher percentages of CO2.
Biosafety Level 1
Quality Control Specifications
Sterility tests are performed on each lot of medium using current USP methods. ATCC also checks each lot of medium for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiencies. Additional test results are listed in the Certificate of Analysis.
Basic Documentation
Other Documentation