Rubella virus (ATCC® VR-315)

Classification: Togaviridae, Rubivirus  /  Product Format: frozen 1 mL per vial

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Classification Togaviridae, Rubivirus
Agent Rubella virus
Strain M33
Common Name Rubella virus
Applications
Respiratory research
Biosafety Level 2
Isolation Throat washings from Army recruit, New Jersey, 1961
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments
Rubella virus is 40-80 nm in diameter and ether sensitive.

Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus.
Effect on Host
No CPE produced on production host. Recognized primarily by interference for other viruses (GMK), or production of CF and HA antigens.
Recommended Host BS-C-1 (ATCC CCL-26)
Growth Conditions Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 11-15 days
Effect on Host
No CPE produced on production host. Recognized primarily by interference for other viruses (GMK), or production of CF and HA antigens.
Name of Depositor PD Parkman, Walter Reed Army Institute of Research
Source Throat washings from Army recruit, New Jersey, 1961
Year of Origin 1961
References

Parkman PD, et al. Recovery of rubella virus from army recruits. Proc. Soc. Exp. Biol. Med. 111: 225-230, 1962. PubMed: 13941530

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation