Human respiratory syncytial virus (ATCC® VR-1540™)

Classification: Paramyxoviridae, Pneumovirus  /  Product Format: frozen 1 mL per vial

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Classification Paramyxoviridae, Pneumovirus
Agent Human respiratory syncytial virus
Strain A2
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images VR-1540 images
Comments
VR-1540 was prepared from ATCC VR-1302 by growth in the presence of neutralizing serum to remove contaminating Adenovirus type 1.
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-10 days
Effect on Host
CPE, syncytial formation, rounding, and sloughing
Name of Depositor ATCC®, L Potash
Special Collection NCRR Contract
Source Lower respiratory tract of infant with bronchiolitis and bronchopneumonia, Melbourne, Australia, 1961. Item treated to remove adenovirus contamination from original deposit.
References

Cameron R, et al. Identification of contaminating adenovirus type 1 in the ATCC reference strain of respiratory syncytial virus A2 (VR-1302). Virus Res. 92: 151-156, 2003. PubMed: 12686423

Lewis FA, et al. A syncytial virus associated with epidemic disease of the lower respiratory tract in infants and young children. Med. J. Aust. 2: 932-933, 1961.

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Micrograph of Host Effects