Human adenovirus 5 (ATCC® VR-1516)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus C  /  Product Format: frozen

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Classification Adenoviridae, Mastadenovirus, Human adenovirus C
Agent Human adenovirus 5
Strain Adenovirus Type 5 Reference Material (The Adenovirus Reference Material (ARM) consists of purified Adenovirus, Type 5 (wild type adenovirus, see ATCC VR-5) formulated as a sterile liquid in 20 mM TRIS, 25 mM NaCl, 2.5% glycerol, pH 8.0 at room temperature, and stored frozen at -70°C. The configuration is 0.5-mL in a Type II glass vial with a Teflon-coated gray butyl stopper, plus an aluminum seal and crimp closure.)
Applications
Reference material
Respiratory research
Positive for Mycoplasma No
Biosafety Level 2
Product Format frozen
Storage Conditions -70°C or colder (Vials have not been tested sufficiently at liquid nitrogen (vapor or liquid) temperatures. At this time it is not certain whether or not the seal will be maintained at cryogenic temperatures.)
Genome Sequenced Strain

Human adenovirus C serotype 5, complete genome.

Comments For additional information on handling the Adenovirus Type 5 Reference Material, view the ARMWG Handling Guide located on the ATCC website at www.atcc.org.
Growth Conditions
Temperature: 37°C
Name of Depositor Adenovirus Reference Material Working Group (ARMWG)
Special Collection NCRR Contract
References

Hutchins B Development of a reference material for characterizing adenovirus vectors Bioprocess. J. 125-28, 2002 Available from: BioProcessing Journal

Hutchins B, et al. Working toward an adenoviral vector testing standard. Mol. Ther. 2: 532-534, 2000. PubMed: 11124052

Nyberg-Hoffman C, et al. Sensitivity and reproducibility in adenoviral infectious titer determination.. Nat. Med. 3: 808-811, 1997. PubMed: 9212113

Smith GJ III, et al. Fast and accurate method for quantitating E. coli host-cell DNA contamination in plasmid DNA preparations.. BioTechniques 26: 518-526, 1999. PubMed: 10090994

Hoffman K. Strategies for host cell protein analysis. BioTechniques 13: 38-45, 2000.

Vallekamp G, et al. Empty capsids in column-purified recombinant adenovirus preparations.. Hum. Gene Ther. 12: 1923-1936, 2001. PubMed: 11589834

Huyghe BG, et al. Purification of a type 5 recombinant adenovirus encoding human p53 by column chromatography.. Hum. Gene Ther. 6: 1403-1416, 1995. PubMed: 8573613

Shabram PW, et al. Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles.. Hum. Gene Ther. 8: 453-465, 1997. PubMed: 9054520

Lehmberg E, et al. Reversed-phase high-performance liquid chromatographic assay for the adenovirus type 5 proteome.. J. Chromatogr. B Biomed. Sci. Appl. 732: 411-423, 1999. PubMed: 10517364

Obenauer-Kutner LJ, et al. The use of field emission scanning electron microscopy to assess recombinant adenovirus stability. Hum. Gene Ther. 13: 1687-1696, 2002. PubMed: 12396622

Callahan JD. Statistical analysis of adenovirus reference material assay results: determination of particle concentration and infectious titer.. La Jolla, CA: Callahan Associates Inc.; 2002.

The Adenovirus Reference Material (ARM) consists of purified Adenovirus, Type 5 (wild type adenovirus, see ATCC VR-5) formulated as a sterile liquid in 20 mM TRIS, 25 mM NaCl, 2.5% glycerol, pH 8.0 at room temperature, and stored frozen at -70°C. The configuration is 0.5-mL in a Type II glass vial with a Teflon-coated gray butyl stopper, plus an aluminum seal and crimp closure.

Vials have not been tested sufficiently at liquid nitrogen (vapor or liquid) temperatures. At this time it is not certain whether or not the seal will be maintained at cryogenic temperatures.

Cross References

Complete Genome (GenBank) : AY339865 Human adenovirus C serotype 5, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation