Vaccinia virus (ATCC® VR-1441)

Classification: Poxviridae, Orthopoxvirus  /  Product Format: frozen

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Classification Poxviridae, Orthopoxvirus
Deposited As Vaccinia virus, Orthopoxvirus
Agent Vaccinia virus
Strain IHD-W
Applications
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s.
The host range includes most mammalian cells and tissues.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no.
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s.
Biosafety Level 2
Isolation
The IHD-W subtype was derived in Japan from IHD-J strain of vaccinia by selection for the ability to induce distinct clear plaques in culture.
Product Format frozen
Storage Conditions -70°C
Comments
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from the IHD-J parent strain, in that IHD-W is unable to induce an active hemagglutinin in the plasma membrane and does elicit polykaryoctosis.
The host range includes most mammalian cells and tissues.
Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).
IHD-W is the strain used by Dales to develop a large group of ts mutants in the 1970s. IHD-W differs from IHD-J in that IHD-W is HA(-) and IHD-J is HA(+).
-70C
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Recommended Host
L2 cells (ATCC CCL-149); chicken embryo fibroblasts

Host of Choice: L2 and CEF

Host Range: most mammalian cells and tissues

most mammalian cells and tissues
Growth Conditions
Duration: 1-5 days at 37C
Cryopreservation
Storage temperature: -70C
Effect on Host
Yes, in vitro effects: Cytopathic effects in tissue culture
CPE
Name of Depositor S Dales
Special Collection NCRR Contract
Source
The IHD-W subtype was derived in Japan from IHD-J strain of vaccinia by selection for the ability to induce distinct clear plaques in culture.
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation