Human adenovirus 1 (ATCC® VR-1)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus C  /  Product Format: frozen 1 mL per vial

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Classification Adenoviridae, Mastadenovirus, Human adenovirus C
Agent Human adenovirus 1
Strain Adenoid 71
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
MRC-5 cells (ATCC® CCL-171™)
Growth Conditions
Temperature: 37°C

Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium

Incubation: 1-4 days

Effect on Host
CPE, refractile rounding and sloughing
Name of Depositor RJ Huebner, National Institute of Allergy and Infectious Diseases
Source Adenoid tissue from five-year-old child with hypertrophied tonsils and adenoids, Maryland, 1953
Year of Origin 1953
References

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Huebner RJ, et al. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. Exp. Biol. Med. 84: 570-573, 1953. PubMed: 13134217

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation