EL4.IL-2 (ATCC® TIB-181)

Organism: Mus musculus, mouse  /  Tissue: thymus  /  Disease: lymphoma

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue
thymus
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain C57BL/6
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
interleukin-2 (interleukin 2, IL-2)
Cellular Products
interleukin-2 (interleukin 2, IL-2)
Comments
This is a subline of EL4 (ATCC TIB-39) that produces IL-2 in response to phorbol-12-myristate-13-acetate (PMA).
The line is capable of producing 2500 units/ml of IL-2 after 24 hours in culture with PMA.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 105 viable cells/mL. Maintain cell density between 1 to 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor JJ Farrar
References

Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789

Cancer therapeutics. New York: Raven Press; 1981.

Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Farrar JJ, et al. Thymoma production of T cell growth factor (interleukin-2). J. Immunol. 125: 2555-2558, 1980. PubMed: 6968789

Cancer therapeutics. New York: Raven Press; 1981.

Fuller-Farrar J, et al. Phorbol myristic acetate enhances the production of interleukin 2. Cell. Immunol. 58: 156-164, 1981. PubMed: 6784931