Renal Epithelial Cell Growth Kit (ATCC® PCS-400-040)

Biosafety Level 1
Product Format frozen 1 kit
Storage Conditions -20°C (or -70°C for long-term storage)
Comments

Renal Epithelial Cell Growth Kit (ATCC PCS-400-040) contains components that when added to Renal Epithelial Cell Basal Medium (ATCC PCS-400-030) create a complete cell culture environment for epithelial cells isolated from normal human kidneys. The low serum (0,5% FBS) medium formulation is designed to support normal renal cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.

The final concentration for each component in complete renal epithelial growth medium is as follows:
Triiodothyronine: 10 nM
rh EGF: 10 ng/mL
Hydrocortisone Hemisuccinate: 100 ng/mL
rh Insulin: 5 µg/mL
Epinephrine: 1 µM
L-Alanyl-L-Glutamine: 2.4 mM
Transferrin: 5 µg/ml Fetal bovine serum (FBS): 0.5%

Volume 1 kit
Sterility Tests
Negative for bacteria, fungi, and yeast.
Functional Tests Rate of proliferation and morphology *A Certificate of Analysis (COA) is available upon request for each lot of Renal Epithelial Cell Growth Kit.
C of A
Certificate of Analysis
Certificate of Analysis