Hs 578Bst ATCC ® HTB-125™ Homo sapiens mammary gland/breast

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	mammary gland/breast
Cell Type	Epithelial, Myoepithelial
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	1
Disease	Carcinoma
Age	74 years
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Karyotype	modal number = 46; range = 42 to 48.. This is a diploid human cell line with 46,XX karyotype. Polyploidy occurred at 6.9%. Both X chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal.
Images
Derivation	Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126).
Clinical Data	74 years Caucasian female
Receptor Expression	Receptor expression: epidermal growth factor (EGF)
Comments	Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo. No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected. A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximately 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze.

Complete Growth Medium	The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%.
Subculturing	Remove medium, and rinse with 0.25% trypsin, 0.53 EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile	Amelogenin: X CSF1PO: 11,13 D13S317: 11,13 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8 vWA: 17
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 0 PGM1, 1 PGM3, 1

Passage History	Note: A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximately 22 more population doublings before the onset of senescence.
References	Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756 Hancock ME, et al. Method for predicting chemosensitivity of anti-cancer drugs. US Patent 4,937,182 dated Jun 26 1990 Stampfer MR, et al. Enhanced growth medium and method for culturing human mammary epithelial cells. US Patent 4,423,145 dated Dec 27 1983

Passage History

Note: A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximately 22 more population doublings before the onset of senescence.

References

Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756

Hancock ME, et al. Method for predicting chemosensitivity of anti-cancer drugs. US Patent 4,937,182 dated Jun 26 1990

Stampfer MR, et al. Enhanced growth medium and method for culturing human mammary epithelial cells. US Patent 4,423,145 dated Dec 27 1983

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph
References	Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756 Hancock ME, et al. Method for predicting chemosensitivity of anti-cancer drugs. US Patent 4,937,182 dated Jun 26 1990 Stampfer MR, et al. Enhanced growth medium and method for culturing human mammary epithelial cells. US Patent 4,423,145 dated Dec 27 1983

Hs 578Bst (ATCC® HTB-125™)

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Hs 578Bst (ATCC^® HTB-125^™)