AN3 CA ATCC ® HTB-111™ Homo sapiens adenocarcinoma

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1
Disease	adenocarcinoma
Age	55 years
Gender	female
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase

Clinical Data	55 years Caucasian female
Tumorigenic	Yes

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation	Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37.0°C

STR Profile	Amelogenin: X CSF1PO: 12,14,15 D13S317: 12,14 D16S539: 10,14 D5S818: 11,14 D7S820: 7,10,7.1 THO1: 10,9.3 TPOX: 8,10 vWA: 14,20
Isoenzymes	AK-1, 1-2 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 1 PGM3, 1-2

Name of Depositor	CJ Dawe
References	Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855 Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 The cells produce undifferentiated malignant tumors. at low frequency (22%) C. J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans.

Name of Depositor

CJ Dawe

References

Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

The cells produce undifferentiated malignant tumors.

at low frequency (22%)

C. J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cancer cell line mutation data
References	Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855 Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 The cells produce undifferentiated malignant tumors. at low frequency (22%) C. J. Dawe and associates derived this cell line from a metastatic lesion in the lymph node of a patient with endometrial carcinoma alerted to the condition by onset of the malignant disorder acanthosis nigricans.

AN3 CA (ATCC® HTB-111™)

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AN3 CA (ATCC^® HTB-111^™)