Ep-16 (ATCC® HB-155)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human epithelium
Cellular Products
immunoglobulin; monoclonal antibody; against human epithelium
Comments
Animals were immunized with a membrane enriched extract of human foreskin keratinocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with an epithelial specific antigen on human foreskin keratinocytes but does not react with mesodermal cells or lymphocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium RPMI 1640 medium, 85%; fetal bovine serum, 15%
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Isotype IgM; kappa light chain
Name of Depositor AW Hamburger
References

Hamburger AW, et al. Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Res. 45: 783-790, 1985. PubMed: 2578308

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hamburger AW, et al. Isolation and characterization of a monoclonal antibody specific for epithelial cells. Cancer Res. 45: 783-790, 1985. PubMed: 2578308