M158 (ATCC® CRL-3086)

Organism: Mus musculus  /  Cell Type: epithelial cell  /  Tissue: breast; mammary gland  /  Disease: breast cancer

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Organism Mus musculus
Tissue breast; mammary gland
Cell Type epithelial cell
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1
Disease breast cancer
Applications

The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.

Storage Conditions liquid nitrogen vapor phase
Images CRL-3086 Micrograph
Derivation The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.
Clinical Data female
Genes Expressed

Myc/c-Myc, expressed

Tumorigenic yes
Comments The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene. 

c-Myc plays a critical role in activating the transcription of growth-related genes. 

Elevated expression of c-Myc has been found in almost one-third of breast and colon carcinomas.  

The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8 X 103 to 1.5 X 104 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C. Subculture when the cell concentration is between 1 X 105 to 2 X 105 cells/cm2.
Subcultivation ratio: A subcultivation ratio of 1:8 to 1:15 is recommended.
Medium renewal: Every 2 to 3 days.
Cryopreservation Freeze medium: complete growth medium supplemented with an additional 10% bovine calf serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C 
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor P Leder
References

Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314

Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-22, 1995 PubMed: 7592814

Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-46, 1998 PubMed:9488037

Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314

Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-22, 1995 PubMed: 7592814

Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-46, 1998 PubMed:9488037

Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823