NU-DUL-1 (ATCC® CRL-2969)

Organism: Homo sapiens, human  /  Cell Type: lymphocyte  /  Tissue: peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid

 /  Disease: undifferentiated lymphoma, non-Burkitt's type

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Organism Homo sapiens, human
Tissue peritonial effusion; derived from metastatic site: malignant cerebrospinal fluid

Cell Type lymphocyte
Product Format frozen
Morphology early B-cell
Culture Properties suspension
Biosafety Level 1
Disease undifferentiated lymphoma, non-Burkitt's type
Age 43 years
Gender male
Ethnicity Caucasian
Applications The Nu-DUL-1 cell line is a valuable resource for studying the biological behavior of undifferentiated lymphoma cells.
The Nu-DUL-1 cell line will aid molecular and cellular studies designed to identify the biological significance of genomic rearrangements.
Storage Conditions liquid nitrogen vapor phase
Karyotype 44, X (hypodiploid), +der(4)t(4;?)(q31;?). -6, -17, -18, -21, +2mar,der(1)t(1;?)(q32;?), del(4)(q25q31), del(5)(p12), del(6)(q21), del(8)(q24), der(11)t(11;?)(q21;?), der(12)t(12;?)(p13;?), der(14)t(14;?)(q13;), ter rea(19;21)(q13;q22)
Images CRL-2969 Micrograph
Clinical Data 43 years
Caucasian
Male
Antigen Expression LN-1+
LN-2+
LN-3+(anti-HLA-Dr)
BA-1+ (CD24)
CALLA (J5)(common ALL antigen; CD10)+
BA-2 - (CD9)
OKM1 - (myeloid marker)
Leu-7 - (natural killer T cell marker)
OKT-3 -, OKT-4 -, OKT-6 -, OKT-8 -, OKT-11 - (T cell markers)
BM-1 -, BM-2 -, BM-3 - ,BM-4 -, BM-5 - (myeloid markers)

EBNA - (Epstein-Barr Nuclear Antigen)
Genes Expressed sIg -
Comments The depositor states that this cell line is negative for the Epstein-Barr Virus (EBV).
ATCC confirmed this cell line is negative for the presence of Epstein-Barr viral DNA sequences via PCR.

The NU-DUL-1 cell line represents a very unusual undifferentiated lymphoma.
The NU-DUL-1 cell line has a chromosome 14 translocation, but not from chromosome 11 or 18 and may represent a new variant.
This cell line is hypodiploid with the presumed loss of a Y chromosome.
NU-DUL-1 cells express markers of early B-cell differentiation.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by the addition of fresh medium. An inoculum of 2.0 x 105to 3.0 x 105cells/mL is recommended. Subculture when the cell concentration is between 8.0 x 105 and 1.0 x 106 cells/mL.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended.
Medium renewal: every 2 to 3 days
Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 12, 14
D13S317: 8, 13
D7S820: 11, 13
D16S539: 11, 13
vWA: 16, 17
THO1: 9, 9.3
Amelogenin: X
TPOX: 8
CSF1PO: 11, 12

Name of Depositor A Epstein
Year of Origin 1985
References

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794