SU-DHL-6 (ATCC® CRL-2959)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: peritoneal effusion; derived from metastatic site: peritoneal cavity  /  Disease: large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma

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Organism Homo sapiens, human
Tissue peritoneal effusion; derived from metastatic site: peritoneal cavity
Cell Type B lymphocyte
Morphology lymphoblast-like
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease large cell lymphoma; diffuse mixed histiocytic and lymphocytic lymphoma; follicular B cell lymphoma
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a pseudodiploid cell line with a modal chromosome number of 47, a polyploidy rate of approximately 9% and one copy of the X chromosome. No Y chromosome was observed in any of the analyzed cells. A derivative t(14;18)(q32;q21) chromosome was present in most of the examined cells. Other derivative chromosomes were generally consistent with previous studies, these include: del(6)(p23), del(9)(p21.1?), add(11)(q25), i(17q) [or t(6?;17)(p10;q10)?] and del(18)(q21)?. [PubMed: 3881165]
Derivation The DHL cell lines were successfully established in continuous suspension culture from 10 patients with a histopathological diagnosis of diffuse histiocytic lymphoma (DHL).
Clinical Data

43 years
Caucasian
male

Genes Expressed monoclonal cytoplasmic immunoglobulin: IgM; lambda light chain
Comments

All of the DHL cell lines were negative for the presence of Epstein-Barr virus (EBV) genomes.
SU-DHL-6 possesses a t(14;18)(q32;q21) translocation and demonstrates an unexpected recombination within its heavy chain gene locus that may be the interchromosomal breakpoint.
In SU-DHL-6, the t(14;18) translocation juxtaposes a truncated bcl-2 gene with J6 in a tail-to-head configuration.

The deregulated expression of the altered bcl-2 gene may play a critical role in the disordered growth and differentiation of follicular B cell lymphoma.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by the addition of fresh medium. An inoculum of 8 X 104 to 3 x 105 cells/mL is recommended. Subculture when cell concentration is between 7 x 105 and 1 x 106 cells/mL.

Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Medium renewal: Every 3 to 4 days

Cryopreservation Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile

D5S818: 12
D13S317: 12,14
D7S820: 10
D16S539: 11,12
vWA: 14,17
THO1: 6,9.3
TPOX: 11,12
CSF1PO: 10
Amelogenin: X

Population Doubling Time approximately 29 hours
Name of Depositor A Epstein
Year of Origin 1977
References

Hua C, et al. Consequences of the t(14;18) chromosomal translocation in follicular lymphoma: deregulated expression of a chimeric and mutated BCL-2 gene. Oncogene Res. 2(3): 263-275, 1988. PubMed: 3285301

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412

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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hua C, et al. Consequences of the t(14;18) chromosomal translocation in follicular lymphoma: deregulated expression of a chimeric and mutated BCL-2 gene. Oncogene Res. 2(3): 263-275, 1988. PubMed: 3285301

Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220

Kaiser-McCaw Hecht B, et al. Histiocytic lymphoma cell lines: immunologic and cytogenetic studies. Cancer Genet. Cytogenet. 14 (3-4): 205-218, 1985. PubMed: 3881165

Siminovitch KA, et al. Immunoglobulin gene rearrangements and expression in diffuse histiocytic lymphomas reveal cellular lineage, molecular defects, and sites of chromosomal translocation. Blood. 67(2): 391-397, 1986. PubMed: 3080039

Epstein AL, Kaplan HS. Feeder layer and nutritional requirements for the establishment and cloning of human malignant lymphoma cell lines. Cancer Res. 39(5):1748-1759, 1979. PubMed: 371794

Bakhshi A, et al. Cloning the chromosomal breakpoint of t(14;18) human lymphomas: clustering around JH on chromosome 14 and near a transcriptional unit on 18. Cell. 41(3):899-906, 1985. PubMed: 3924412