BSC40 [BSC-40] (ATCC® CRL-2761)

Organism: Cercopithecus aethiops  /  Cell Type: epithelial  /  Tissue: kidney  / 

Permits and Restrictions

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Organism Cercopithecus aethiops
Tissue kidney
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Applications This cell line is a suitable viral host.
Storage Conditions liquid nitrogen vapor phase
Derivation BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans.
Virus Susceptibility Vaccinia virus
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.
  5. Add appropriate aliquots of cell suspension to new culture vessels.
  6. Incubate culture vessels at 37°C.

Subcultivation Ratio: 1:5 to 1:10
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Max Temperature: 40°C
Min Temperature: 31°C
Name of Depositor RC Condit
References

Condit RC, Motyczka A. Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113: 224-241, 1981. PubMed: 7269240

Brockman WW, Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc. Natl. Acad. Sci. USA 71: 942-946, 1974. PubMed: 4362640

Ensinger MJ. Isolation and genetic characterization of temperature-sensitive mutants of vaccinia virus WR. J. Virol. 43: 778-790, 1982. PubMed: 7143565

BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31C to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies.

Basic Documentation
Restrictions

This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit

References

Condit RC, Motyczka A. Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113: 224-241, 1981. PubMed: 7269240

Brockman WW, Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc. Natl. Acad. Sci. USA 71: 942-946, 1974. PubMed: 4362640

Ensinger MJ. Isolation and genetic characterization of temperature-sensitive mutants of vaccinia virus WR. J. Virol. 43: 778-790, 1982. PubMed: 7143565

BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31C to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies.