Het-1A ATCC ® CRL-2692™ Homo sapiens esophagus normal

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Homo sapiens, human
Tissue	esophagus
Cell Type	epithelial; SV40 large T antigen transfected
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	2 [cells contain SV40 viral sequences]
Disease	normal
Age	25 years
Gender	male
Ethnicity	Black
Applications	The cell line may be useful for investigating the action of putative esophageal carcinogens.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	hypodiploid (34-40 chromosomes) RefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038
Derivation	The HET-1A cell line was derived in 1986 from human esophageal autopsy tissue by transfection with plasmid pRSV-T consisting of the RSV-LTR promoter and the sequence encoding the simian virus 40 large T-antigen.
Clinical Data	25 years Black male
Antigen Expression	SV40 T antigen RefStoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038RefTolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621
Genes Expressed	cytokeratin,SV40 T antigen
Tumorigenic	No
Comments	The line has undergone more than 250 population doublings. Growth factor studies have shown that HET-1A is stimulated by calcium and inhibited by fetal bovine serum, transforming growth factor-beta 1, and transforming growth factor-beta 2. Fumonisin B1 produces growth inhibition and increased apoptosis in HET-1A cells. The synthetic retinoid CD437 (6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (AHPN/CD437)0 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway.

Complete Growth Medium	The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit. Note: Do not filter complete medium.
Subculturing	Remove the spent medium, and add 0.05% trypsin -0.53 mM EDTA solution containing 0.5% polyvinylpyrrolidone (PVP) and allow the culture to sit at room temperature until the cells begin to detach (usually 5 to 10 minutes). Neutralize the trypsin with 0.1% soybean trypsin inhibitor. Resuspend the cells in fresh medium, aspirate and dispense into coated flasks. The flasks used should be precoated with with a mixture of 0.01 mg/mL fibronectin, 0.03 mg/mL bovine collagen type I and 0.01 mg/mL bovine serum albumin dissolved in culture medium. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Leibovitz's L-15 medium with 2 mM L-glutamine and 10mM HEPES supplemented with 1% PVP, 10% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C

STR Profile	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 9,11 D5S818: 11,12 D7S820: 9 THO1: 7 TPOX: 11 vWA: 16

Name of Depositor	CC Harris
Year of Origin	1986
References	Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 Milo GE, et al. Conversion of premalignant human cells to tumorigenic cells by methylmethane sulfonate and methylnitronitrosoguanidine. Cell Biol. Toxicol. 8: 193-205, 1992. PubMed: 1337307 Tolleson WH, et al. Apoptotic and anti-proliferative effects of fumonisin B1 in human keratinocytes, fibroblasts, esophageal epithelial cells and hepatoma cells. Carcinogenesis 17: 239-249, 1996. PubMed: 8625445 Tolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621 Wan X, et al. Synthetic retinoid CD437 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. Anticancer Res. 21: 2657-2663, 2001. PubMed: 11724335 Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038

Name of Depositor

CC Harris

Year of Origin

1986

References

Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038

Milo GE, et al. Conversion of premalignant human cells to tumorigenic cells by methylmethane sulfonate and methylnitronitrosoguanidine. Cell Biol. Toxicol. 8: 193-205, 1992. PubMed: 1337307

Tolleson WH, et al. Apoptotic and anti-proliferative effects of fumonisin B1 in human keratinocytes, fibroblasts, esophageal epithelial cells and hepatoma cells. Carcinogenesis 17: 239-249, 1996. PubMed: 8625445

Tolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621

Wan X, et al. Synthetic retinoid CD437 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. Anticancer Res. 21: 2657-2663, 2001. PubMed: 11724335

Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Restrictions	The cells are distributed for research purposes only. The National Cancer Institute (NCI) releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Neither the cell line nor products derived from it may be sold or used for commercial purposes. 2.) Any commercial requests or proposed commercial use of these cells must first be negotiated with the Office of Technology Transfer, NIH, 6011 Executive Blvd., Room 325, Rockville, MD 20852; Telephone (301) 496-7057; FAX (301) 402-0220
References	Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038 Milo GE, et al. Conversion of premalignant human cells to tumorigenic cells by methylmethane sulfonate and methylnitronitrosoguanidine. Cell Biol. Toxicol. 8: 193-205, 1992. PubMed: 1337307 Tolleson WH, et al. Apoptotic and anti-proliferative effects of fumonisin B1 in human keratinocytes, fibroblasts, esophageal epithelial cells and hepatoma cells. Carcinogenesis 17: 239-249, 1996. PubMed: 8625445 Tolleson WH, et al. The mycotoxin fumonisin induces apoptosis in cultured human cells and in livers and kidneys of rats. Adv. Exp. Med. Biol. 392: 237-250, 1996. PubMed: 8850621 Wan X, et al. Synthetic retinoid CD437 induces apoptosis of esophageal squamous HET-1A cells through the caspase-3-dependent pathway. Anticancer Res. 21: 2657-2663, 2001. PubMed: 11724335 Stoner GD, et al. Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells. Cancer Res. 51: 365-371, 1991. PubMed: 1703038

Het-1A (ATCC® CRL-2692™)

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Het-1A (ATCC^® CRL-2692^™)