MES-SA/MX2 (ATCC® CRL-2274)

Organism: Homo sapiens, human  /  Disease: uterine sarcoma

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Organism Homo sapiens, human
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease uterine sarcoma
Age 56 years
Gender female
Ethnicity Caucasian
Applications
The clone, designated MES-SA/MX2, was approximately 975 fold less sensitive to mitoxantrone than the parental cells.
Derivation
MES-SA/MX2 is a mitoxantrone resistant derivative of the human uterine sarcoma cell line MES-SA (see ATCC CRL-1976).
Clinical Data
female
Caucasian
56 years
Comments
MES-SA/MX2 is a mitoxantrone resistant derivative of the human uterine sarcoma cell line MES-SA (see ATCC CRL-1976).
The line was selected and subcloned in 1988 for resistance to mitoxantrone, an anthracenedione antitumor agent.
These cells were cloned by limiting dilution in soft agar, propagated and tested for sensitively to mitoxantrone.
The clone, designated MES-SA/MX2, was approximately 975 fold less sensitive to mitoxantrone than the parental cells.
The cells display features of both classic multidrug resistance (MDR), P-glycoprotein overexpression and atypical MDR, altered topoisomerase II catalytic activity.
Complete Growth Medium A 1:1 mixture of Waymouth's MB 752/1 medium and McCoy's 5a medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
These cells grow as a mixture of floating and adherent cells.
Remove the medium with the floating cells, and recover the cells by centrifugation.
Rinse the adherent cells with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution, and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate, combine with the floating cells recovered above and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,12
vWA: 18
Name of Depositor WG Harker
References

Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344

Harker WG, Sikic BI. Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344

Harker WG, Sikic BI. Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002