RS4;11 (ATCC® CRL-1873™)

Organism: Homo sapiens, human  /  Tissue: bone marrow  /  Cell Type: lymphoblast

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Organism Homo sapiens, human
Tissue
bone marrow
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute lymphoblastic leukemia
Age 32 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype The cells have a characteristic chromosomal abnormality, t(4;11)(q21;q23), and an isochromosome for the long arm of chromosome 7.
Derivation
This line was established from the bone marrow of a patient with acute lymphoblastic leukemia.
Clinical Data
32 years
Caucasian
female
Antigen Expression
HLA DR +; CD9 +; CD24 +
Genes Expressed
HLA DR +; CD9 +; CD24 +
Comments The cells lack surface and cytoplasmic immunoglobulin, and are negative for CALLA (CD10).

The cells are also negative for myeloperoxidase and chloroacetate esterase and do not stain with Sudan Black. The cells are strongly positive for terminal deoxynucleotidyl transferase (TdT) and are positive for BA-1 (CD24), BA-2 (CD9) and MHC Class II antigens (HLA DR).

 
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 105 viable cells/mL. Maintain cell density between 1 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 14,17
Name of Depositor JH Kersey
References

Stong RC, et al. Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics. Blood 65: 21-31, 1985. PubMed: 3917311

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Leukemia cell line mutation data
References

Stong RC, et al. Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics. Blood 65: 21-31, 1985. PubMed: 3917311