RL95-2 (ATCC® CRL-1671)

Organism: Homo sapiens, human  /  Tissue: uterus/endometrium  /  Disease: carcinoma

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Organism Homo sapiens, human
Tissue uterus/endometrium
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease carcinoma
Age 65 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
65 years
Caucasian
female
Receptor Expression
estrogen receptor, positive
Comments
The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli.
Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • 0.005 mg/ml insulin
  • fetal bovine serum to a final concentration of 10%

Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,12
D16S539: 11,13
D5S818: 10,11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 16,20
Name of Depositor DL Way
References

Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371

Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Way DL, et al. Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture. In Vitro 19: 147-158, 1983. PubMed: 6339371

Cohen LA. Isolation and characterization of a serially cultivated, neoplastic, epithelial cell line from the N-nitrosomethylurea induced rat mammary adenocarcinoma. In Vitro 18: 565-575, 1982. PubMed: 7118137

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Yang N, et al. Estrogen-related receptor, hERR1, modulates estrogen receptor-mediated response of human lactoferrin gene promoter. J. Biol. Chem. 271: 5795-5804, 1996. PubMed: 8621448