Human genomic DNA (RWPE-2) (ATCC® CRL-11610D™)

Organism: Homo sapiens, human  / 

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Format frozen Approx.10 µg
Storage Conditions -20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level 2
Introduction

Cell Line Description:

Epithelial cells from a histologically normal adult human prostate were isolated and subsequently transfected with a plasmid carrying one copy of the human papillomavirus 18 (HPV-18) genome to establish the RWPE-1 (ATCC CRL-11609) cell line.

Cells from the RWPE-1 cell line were further transformed by Ki-ras using the Kirsten murine sarcoma virus (Ki-MSV) to establish the RWPE-2 cell line (ATCC CRL-11610). RWPE-2 cells form colonies in agar and tumors in nude mice.

See www.atcc.org for more information
Quality Control Specifications

DNA Quality Control Information:

·         > 100 kb in size by agarose gel electrophoresis

·         No RNA detected in the agarose gel

·         A260/A280 ratio ³ 1.8

·         Tested and verified for PCR amplification and restriction digestion.
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS