NC-37 (ATCC® CCL-214)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Disease: Burkitt's lymphoma

Organism Homo sapiens, human
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain HERPESVIRUS
Disease Burkitt's lymphoma
Gender male
Applications
The NC-37 line has been utilized as a target cell for EBV superinfection studies and has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses.
The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies.
lthough deposited with the ATCC as a lymphoblastoid cell line established from a 34 year old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitt's lymphoma cell line.
Karyotype modal number = 48; range = 40 to 51
Derivation
Maywood New Jersey, United States
Clinical Data
BR>Although deposited with the ATCC as a lymphoblastoid cell line established from a 34 year old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitt's lymphoma cell line.
male
Comments
The NC-37 line has been utilized as a target cell for EBV superinfection studies and has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses. The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies.
Each cell contains an average of 60 EBV genome copies. The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-).
Although deposited with the ATCC as a lymphoblastoid cell line established from a 34 year old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitt's lymphoma cell line.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 5 X 10(5) viable cells/ml. A maximum density of upto 2 X 10(6) is possible.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 3 to 4 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO.
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13,14
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16
Isoenzymes
G6PD, B
Name of Depositor J Wolff
Year of Origin February, 1967
References

Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017

Mayyasi SA, et al. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499

The NC-37 cell line was reportedly initiated by W. Korol from peripheral blood from a donor whose serum was positive for EBV antibodies as determined by immunofluorescence and virus coating test.

Basic Documentation
References

Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017

Mayyasi SA, et al. The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499

The NC-37 cell line was reportedly initiated by W. Korol from peripheral blood from a donor whose serum was positive for EBV antibodies as determined by immunofluorescence and virus coating test.