HeLa 229 (ATCC® CCL-2.1)

Organism: Homo sapiens, human  /  Tissue: cervix  /  Disease: adenocarcinoma

Organism Homo sapiens, human
Tissue cervix
Product Format frozen
Morphology epithelial
Culture Properties adherent (The line can be readily adapted to growth in suspension)
Biosafety Level 2 [Cells contain human papilloma virus (HPV-18)]
Disease adenocarcinoma
Age 31 years
Gender female
Ethnicity Black
Storage Conditions liquid nitrogen vapor phase
Clinical Data
31 years
Black
female
HeLa Markers Y
Cellular Products
keratin
Virus Susceptibility Human adenovirus 7
Vesicular stomatitis virus
Virus Resistance Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Comments
Relatively resistant to polioviruses.

The cells are positive for keratin by immunoperoxidase staining.

This cell line can be readily adapted to growth in suspension.

ATCC confirmed this cell line is positive for the presence of Papillomavirus viral DNA sequences via PCR.

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor JT Syverton
References

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

The line can be readily adapted to growth in suspension

Basic Documentation
References

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

The line can be readily adapted to growth in suspension