BAA-562
BAA-562 ™
BAA-562 ™
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. If needed exchange the gas in the test tube for 100% N2, 80% N2-20%CO2. When thiosulfate is the electron donor the culture can be grown under aerobic conditions (N2-CO2-O2, 65:15:20).
3. When the Balch tube is ready to inoculate, open the vial according to enclosed instructions.
4. For inoculation, use a 1.0 ml syringe tipped with 22 gauge needle. Withdraw 0.5 ml of Medium #2346 and use this to rehydrate the freeze-dried pellet. Transfer the rehydrated cell suspension back to a tube of #2346 broth and incubate at 60oC. Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37oC. Inoculate a nonselective anaerobic and aerobic broth and incubate at 60oC
5. Growth should be detected in the #2346 broth within 24 to 48 hours. There should be no growth detected on the aerobic plate. There should be no growth in the nonselective aerobic or anaerobic broth.
Growth will be detected within 24 to 48 hours by turbidity that settles at the bottom of the test tube. The turbidity of the culture increases form 24 to 48 hours but the cell density does not.
The cells are Gram negative motile rods with a polar flagella. The cells are typically in short chains.
Once growth has been established, the culture should be transferred every 24 to 48 hours when maintained at 60oC. The culture can be maintained at 4oC for up to 1 week..
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Resolution: 1000X
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Takai K, et al. Isolation and metabolic characteristics of previously uncultured members of the order aquificales in a subsurface gold mine.. Appl. Environ. Microbiol. 68: 3046-3054, 2002. PubMed: 12039766
type strain
Takai K, et al. Sulfurihydrogenibium subterraneum gen. nov., sp. nov., from a subsurface hot aquifer. Int. J. Syst. Evol. Microbiol. 53: 823, 2003. PubMed: 12807207