pWE4 phagemid in E. coli (ATCC® 87674)

Applications: expression vector  /  Depositors: M Cockett

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Designations pWE4 phagemid in E. coli
Depositors M Cockett
Biosafety Level 1
Vector Information
Size (kb): 6.511
Vector: pWE4 (phagemid)
Promoters: Promoter for in vitro transcription SP6
Marker(s):ampR,hygR
Construct size (kb): 6.511
Features: marker(s): ampR
marker(s): hygR
promoter for expression: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40
replicon: f1
replicon: pMB1
MCS: HindIII...ApaI
terminator: SV40 polyadenylation
enhancer: SV40
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, 1.6; SalI--3.6, 2.8.
Having identical cloning sites also facilitates shuttling cDNAs from one vector to another.
One of a series of vectors (ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678).
Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206

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component of:ATCC 87678
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