pWE2 (ATCC® 87672)

Applications: Expression vector  /  Depositors: M Cockett

Permits and Restrictions

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Designations pWE2
Depositors M Cockett
Biosafety Level 1
Host Escherichia coli HB101, ATCC 33694
Vector Information
Size (kb): 7.014
Vector: pWE2 (phagemid)
Promoters: Promoter for in vitro transcription SP6
Marker(s): G418R,ampR
Construct size (kb): 7.014
Features: marker(s): G418R
marker(s): ampR
promoter for expression: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40
replicon: f1
replicon: pMB1
MCS: HindIII...ApaI
terminator: SV40 polyadenylation
enhancer: SV40
Applications
Expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; SalI--7.0.
Having identical cloning sites also facilitates shuttling cDNAs from one vector to another.
One of a series of vectors (ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678).
Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206

Related Products
Component of: ATCC 87678
Shipped frozen
Shipping Information

 Frozen glycerol stock of  E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87672