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pTATALUC+

87631

Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
reporter construct
shuttle vector

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; HindIII--4.6; EcoRI--4.6.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The vector pTATALUC+ carries only the TATA box to avoid interference of the tk-derived CAAT and GC boxes with the regulatory elements of interest.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.64300012588501
Intact vector size
4.643
Vector name
pTATALUC+ (plasmid)
Type of vector
plasmid
Host range
vertebrate cells
Cloning sites
HindIII; PstI; SalI; BamHI; ClaI; SmaI; KpnI; SacI; EcoRI
Markers
ampR
MCS
HindIII...BamHI, ->, 1-36
Polylinker sites
HindIII; PstI; SalI; BamHI; [tk-promoter luc+ SV40 poly (A)] ClaI; SmaI; KpnI; SacI; EcoRI
Promoters
truncated TK promoter, 37-131
Replicon
pMB1, 2519-2519
Reporter group
luciferase (luc+), ->, 170-1822
Terminator
SV40 late polyadenylation, 1854-2075; SV40 large T-antigen polyadenylation, 4269-4640

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI--4.6;
HindIII--4.6; EcoRI--4.6.
- ATCC staff

The vector contains modified luciferase (luc+) gene from the firefly Photinus
pyralis in order to increase the yield of recoverable luciferase activity after
transfection and to eliminate potential cryptic regulatory elements.
- Biotechniques 23: 436-438, 1997

The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for
the characterization of isolated cis regulatory elements.
- Biotechniques 23: 436-438, 1997

The promoters driving luc+ transcription in these two vectors represent
different 5' deletions of the tk promoter ending at -32 and -105, respectively.
- Biotechniques 23: 436-438, 1997

The vector pTATALUC+ carries only the TATA box to avoid interference of the
tk-derived CAAT and GC boxes with the regulatory elements of interest.
- Biotechniques 23: 436-438, 1997

The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide
valuable tools for the analysis of eukaryotic regulatory DNA elements.
- Biotechniques 23: 436-438, 1997

History

Depositors
J Duschl, J Altschmied
Special collection
NCRR Contract
Cross references
GenBank AF027127

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214

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