pTATALUC+ (ATCC® 87631)

Applications: reporter constructshuttle vector  /  Depositors: J Duschl, J Altschmied

Permits and Restrictions

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Designations pTATALUC+
Depositors J Duschl, J Altschmied
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.6430001258850100
DESCRIPTION OF VECTOR:
Intact vector size: 4.643
Type of vector: plasmid
Cloning sites: HindIII PstI SalI BamHI ClaI SmaI KpnI SacI EcoRI
Polylinker sites: HindIII PstI SalI BamHI [tk-promoter luc+ SV40 poly (A)]ClaI
SmaI KpnI SacI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: truncated TK promoter, 37-131
reporter group: luciferase (luc+), ->, 170-1822
terminator: SV40 late polyadenylation, 1854-2075
MCS: ClaI...EcoRI, 2078-2106
replicon: pMB1, 2519-2519
marker(s): ampR, <-, 3277-4137
terminator: SV40 large T-antigen polyadenylation, 4269-4640
Vector: pTATALUC+ (plasmid)
Promoters: Promoter truncated TK promoter
Marker(s):ampR
Construct size (kb): 4.64300012588501
Features: marker(s): ampR
promoter: truncated TK promoter
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...EcoRI
MCS: HindIII...BamHI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Applications
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.6; HindIII--4.6; EcoRI--4.6.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The vector pTATALUC+ carries only the TATA box to avoid interference of the tk-derived CAAT and GC boxes with the regulatory elements of interest.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Altschmied J, Duschl J. Set of optimized luciferase reporter gene plasmids compatible with widely used CAT vectors. BioTechniques 23: 436-438, 1997. PubMed: 9298214

Related Products
component of:ATCC 87654
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
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