pSLF104 (ATCC® 87621)

Applications: YI-type (integrating) shuttle vector  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations pSLF104
Depositors SL Forsburg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.8000001907348630
Vector: pSLF104 (plasmid)
Promoters: Promoter adh
Construct size (kb): 4.800000190734863
Features: marker(s): ampR
marker(s): sup3-5
promoter: adh
repressor gene: tet
MCS: HindIII...EcoRI
YI-type (integrating) shuttle vector
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.4, 0.58; BamHI--2.9, 1.9; EcoRI--3.6, 1.2.
The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter.
When integrated into a strain of genotype h- ade6-704 leu1-32 ura4-D18, the resulting fission yeast strain, FY191 (ATCC ) produces a constitutive tetracycline repressor protein.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

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