pEA500 (ATCC® 87618)

Applications: YE-type (episomal) shuttle vector  /  Depositors: SL Forsburg

Permits and Restrictions

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Designations pEA500
Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 8.3000001907348630
Vector: pEA500 (plasmid)
Promoters: Promoter T3
Construction: pEA2, pSP2
Marker(s):ampR,his7+
Construct size (kb): 8.300000190734863
Features: insert detection: lacZ'
marker(s): ampR
marker(s): his7+
promoter: T7, T3
replicon: ars1
replicon: pMB1
MCS: KpnI...SacII
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--8.3; XhoI--8.3; HindIII--5.3, 1.6, 1.4.
Plasmid pEA500 is a cloning vector that carries his7+ and ars1, yet retains the ability to use the blue/white color screen to identify recombinants.
The vector was constructed by inserting the PstI-SmaI fragment of pEA2 into plasmid pSP2 digested with NsiI and StuI (both recognize unique sites within the URA3 gene).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Apolinario E, et al. Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491-495, 1993. PubMed: 8299169

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