REP3X (ATCC® 87603)

Applications: expression vector  /  Depositors: SL Forsburg

Permits and Restrictions

View Permits

Designations REP3X
Depositors SL Forsburg
Biosafety Level 1
Vector Information
Size (kb): 8.8999996185302730
Vector: REP3X (phagemid)
Promoters: Promoter for expression nmt1 (full strength)
Construction: pUC119
Marker(s):LEU2,ampR
Construct size (kb): 8.899999618530273
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: nmt1 (full strength)
replicon: ars1
replicon: f1
replicon: pMB1
MCS: Xho...SmaI
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.6, 2.3, 1.1; EcoRI--4.3, 3.5, 1.2; XhoI--9.0.
REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**)
due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993).
REP3X (ATCC 87603) and REP4X (ATCC 87604) differ in that REP3X contains LEU2 (2.2 kb) and REP4X contains ura4+ (1.8 kb).
REP3X was constructed by removing an ATG within the polylinker of an expression vector REP3, which contains the thiamine-inducible nmt1 promoter (J. Biol. Chem. 265:10857-10862, 1993), by insertion of a XhoI linker into Ball and SalI sites.
The SalI site is preserved.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Susan L Forsburg, personal communication

Related Products
component of:ATCC 87685
Shipped frozen
Product Sheet
Product Sheet
Product Sheet