YEplac112 (ATCC® 87590)

Applications: YE-type (episomal) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Designations YEplac112
Depositors RD Gietz
Biosafety Level 1
Vector Information
Size (kb): 4.989
Vector: YEplac112 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):TRP1,ampR
Construct size (kb): 4.989
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI
Applications
YE-type (episomal) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
This is a YE-type (yeast episomal) vector with the TRP1 marker.
The HindIII, PstI and the XbaI sites from the TRP1 gene of the S. cerevisiae were removed by using oligo-directed mutagenesis.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
Restriction digests of the clone give the following sizes (kb): AvaI--2.9, 1.3, 0.8; HindIII--4.9; PstI--4.9.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

Related Products
component of:ATCC 87623
component of:ATCC 87638
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87590