YEplac181 (ATCC® 87588)

Applications: YE-type (episomal) shuttle vectorvector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase  /  Depositors: RD Gietz

Designations YEplac181
Depositors RD Gietz
Biosafety Level 1
Vector Information
Size (kb): 5.7410001754760740
Vector: YEplac181 (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):LEU2,ampR
Construct size (kb): 5.741000175476074
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
replicon: 2 micron ori
replicon: pMB1
MCS: HindIII...EcoRI
Applications
YE-type (episomal) shuttle vector
vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--5.2, 0.8; ClaI--6.0; EcoRI--6.0.
One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1).
The EcoRI and KpnI restriction sites from the LEU2 gene of S. cerevisiae were removed by using oligo-directed mutagenesis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gietz RD, Sugino A. New yeast Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534, 1988. PubMed: 3073106

Related Products
component of:ATCC 87623
component of:ATCC 87638
Shipped frozen
Shipping Information

 Freeze dried Escherichia coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87588