pRS415 (ATCC® 87520)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

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Designations pRS415
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 6.02
Vector: pRS415 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):LEU2,ampR
Construct size (kb): 6.02
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--5.55, 0.45; BglI--4.3, 1.7; ClaI--4.45, 1.55.
A YC-type shuttle vector with LEU2 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Related Products
component of:ATCC 87533
Shipping Information

 Frozen glycerol stock of phagemid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87520