pRS412 (ATCC® 87478)

Applications: YC-type (centromeric) shuttle vectorvector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase  /  Depositors: JD Boeke

Permits and Restrictions

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Designations pRS412
Depositors JD Boeke
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.042
DESCRIPTION OF VECTOR:
Intact vector size: 6.042
Type of vector: phagemid
Cloning sites: SacI SacII EagI NotI SpeI BamHI SmaI PstI EcoRI ClaI SalI
XhoI ApaI KpnI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pRS402, pRS411
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ADE2, ->, 575-2290
replicon: f1, <-, 2495-2951
promoter for in vitro transcription: T7, ->, 3121-3140
MCS: KpnI...SacI, ->, 3148-3250
promoter for in vitro transcription: T3, <-, 3267-3286
insert detection: lacZ', <-, 2952-3311
promoter: lac, <-, 3356-3384
replicon: pMB1, 3710-3710
marker(s): ampR, <-, 4468-5328
centromere: CEN6, 5470-5586
replicon: ARS4, 5587-5988
Vector: pRS412 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRS402, pRS411
Marker(s):ampR,ADE2
Construct size (kb): 6.042
Features: insert detection: lacZ'
marker(s): ADE2
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARS4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
vector permitting visual detection of recombinants phosphoribosylaminoimidazole succinocarboxamide synthetase SAICAR synthetase, phosphoribosylaminoimidazole carboxylase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0; BglII--2.2, 3.8; EcoRI--6.0.
ade2 phenotype produces red colonies when grown on adenine containing media.
This vector is useful for gene knockout experiments in hosts with a non-revertable ade2 auxotrophic marker gene mutation.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

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Shipped frozen
Shipping Information Distributed: freeze-dried
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