p426 CYC1 (ATCC® 87385)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p426 CYC1
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.244
Vector: p426 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS426 (ATCC 77107)
Construct size (kb): 6.244
Features: marker(s): URA3
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
terminator: CYC1
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.75, 0.35; XbaI--3.4, 2.9; EcoRI--6.3.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information

 Freeze dried E. coli containing the plasmid.

Product Sheet
Product Sheet
Product Sheet
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