p426 CYC1 (ATCC® 87385)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

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Designations p426 CYC1
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.244
Vector: p426 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS426 (ATCC 77107)
Marker(s):URA3,ampR
Construct size (kb): 6.244
Features: marker(s): URA3
marker(s): ampR
promoter for expression: CYC1
replicon: 2 micron
MCS: SalI...XBAI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.75, 0.35; XbaI--3.4, 2.9; EcoRI--6.3.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.

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