p416 CYC1 (ATCC® 87384)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p416 CYC1
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.4109997749328610
DESCRIPTION OF VECTOR:
Intact vector size: 5.411
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS416
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1323
terminator: CYC1, ->, 2005-2265
MCS: SalI...XbaI, ->, 2271-2328
promoter for expression: CYC1, <-, 2329-2616
marker(s): ampR, <-, 3863-4793
replicon: CEN6/ARSH4, ->, 4887-5391
Vector: p416 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS416
Marker(s):URA3,ampR
Construct size (kb): 5.410999774932861
Features: marker(s): URA3
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 0.3; KpnI--5.4; EcoRI--5.4.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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