p414 CYC1 (ATCC® 87380)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p414 CYC1
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.3010001182556150
DESCRIPTION OF VECTOR:
Intact vector size: 5.301
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: CEN6/ARSH4, ->
marker(s): TRP1, ->
terminator: CYC1, ->
promoter for expression: CYC1, <-
marker(s): ampR, <-
Vector: p414 CYC1 (plasmid)
Promoters: Promoter for expression CYC1
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 5.301000118255615
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CYC1
replicon: CEN6/ARSH4
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; EcoRI--5.6; SacI/XbaI--3.4, 1.7.
Contains a truncated version of the CYC1 promoter that very weak and no longer inducible because of a deletion of most of the UAS2 sequence.
Low copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information Distributed: freeze-dried
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