p426 ADH (ATCC® 87377)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p426 ADH
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 7.4569997787475590
DESCRIPTION OF VECTOR:
Intact vector size: 7.457
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
promoter for expression: ADH, <-, 2339-3839
marker(s): ampR, <-, 4958-5888
replicon: 2 micron, ->, 5987-7327
Vector: p426 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS426 (ATCC 77107)
Marker(s):URA3,ampR
Construct size (kb): 7.456999778747559
Features: marker(s): URA3
marker(s): ampR
promoter for expression: ADH
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.8, 1.5; EcoRI--7.4; XbaI--4.6, 2.7.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
High copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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