p416 ADH (ATCC® 87376)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p416 ADH
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.6240000724792480
Vector: p416 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS416
Marker(s):URA3,ampR
Construct size (kb): 6.624000072479248
Features: marker(s): URA3
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 1.5; KpnI--6.6; EcoRI--6.6.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
Low copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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Attachments Vector map for 87376