p416 ADH (ATCC® 87376)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p416 ADH
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.6240000724792480
Vector: p416 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS416
Marker(s):URA3,ampR
Construct size (kb): 6.624000072479248
Features: marker(s): URA3
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.1, 1.5; KpnI--6.6; EcoRI--6.6.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information

 Freeze dried phagemid in E. coli

Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 87376