p414 ADH (ATCC® 87372)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p414 ADH
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.514
DESCRIPTION OF VECTOR:
Intact vector size: 6.514
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...XbaI, ->, 2155-2219
promoter for expression: ADH, <-, 2219-3719
marker(s): ampR, <-, 4966-5896
replicon: CEN6/ARSH4, ->, 6001-6514
Vector: p414 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 6.514
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; XhoI--6.6; SacI/XbaI--3.2, 1.8, 1.5.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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