p414 ADH (ATCC® 87372)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p414 ADH
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.514
DESCRIPTION OF VECTOR:
Intact vector size: 6.514
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...XbaI, ->, 2155-2219
promoter for expression: ADH, <-, 2219-3719
marker(s): ampR, <-, 4966-5896
replicon: CEN6/ARSH4, ->, 6001-6514
Vector: p414 ADH (plasmid)
Promoters: Promoter for expression ADH
Construction: pRS414
Marker(s):TRP1,ampR
Construct size (kb): 6.514
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; XhoI--6.6; SacI/XbaI--3.2, 1.8, 1.5.
The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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