p426 TEF (ATCC® 87369)

Applications: YX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p426 TEF
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.3590002059936520
DESCRIPTION OF VECTOR:
Intact vector size: 6.359
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS426 (ATCC 77107)
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 200-1300
terminator: CYC1, ->, 2004-2264
MCS: XhoI...XbaI, ->, 2265-2339
promoter for expression: TEF, <-, 2339-2741
marker(s): ampR, <-, 3859-4789
replicon: 2 micron, ->, 4889-6229
Vector: p426 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS426 (ATCC 77107)
Marker(s):URA3,ampR
Construct size (kb): 6.359000205993652
Features: marker(s): URA3
marker(s): ampR
promoter for expression: TEF
replicon: 2 micron
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.5; SacI/XBaI--3.2, 2.7, 0.44; XhoI--6.5.
High copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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