p425 TEF (ATCC® 87367)

Applications: YE-type (episomal) shuttle vectorYX-type (expression) shuttle vector  /  Depositors: M Funk

Permits and Restrictions

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Designations p425 TEF
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.482
Vector: p425 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS425
Marker(s):LEU2,ampR
Construct size (kb): 7.482
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: TEF
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Applications
YE-type (episomal) shuttle vector
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.0, 2.4; SacI/XbaI--3.8, 3.2, 0.42.
High copy number shuttle expression vector.
this vector has the TEF promoter for expression, the LEU2 marker gene and the 2 micron replicon. It is distributed in E. coli.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Shipping Information

 Freeze dried E. coli containing the plasmid

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