p415 TEF (ATCC® 87366)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

View Permits

Designations p415 TEF
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.6490001678466800
Vector: p415 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS415
Marker(s):LEU2,ampR
Construct size (kb): 6.64900016784668
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; SacI/XbaI--6.2, 0.42; XhoI--6.6.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
component of:ATCC 87669
Shipped frozen
Product Sheet
Product Sheet
Product Sheet