p413 TEF (ATCC® 87362)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p413 TEF
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5980000495910650
Vector: p413 TEF (plasmid)
Promoters: Promoter for expression TEF
Construction: pRS413
Construct size (kb): 5.598000049591065
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: TEF
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; SacI/XbaI--5.2, 0.4; XhoI--5.6; EcoRI--5.6.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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