p415 GPD (ATCC® 87358)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

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Designations p415 GPD
Depositors M Funk
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 6.9270000457763670
DESCRIPTION OF VECTOR:
Intact vector size: 6.927
Type of vector: plasmid
Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS415
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, ->, 200-2446
terminator: CYC1, ->, 3128-3388
MCS: XhoI...XbaI, ->, 3388-3452
promoter for expression: GPD, <-, 3452-4132
marker(s): ampR, <-, 5379-6309
replicon: CEN6/ARSH4, ->, 6141-6927
Vector: p415 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS415
Marker(s):LEU2,ampR
Construct size (kb): 6.927000045776367
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--6.2, 0.7; EcoRI--4.6, 2.3; BamHI--6.9.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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component of:ATCC 87669
Shipped frozen
Shipping Information Distributed: freeze-dried