p413 GPD (ATCC® 87354)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

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Designations p413 GPD
Depositors M Funk
Biosafety Level 1
Host Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 5.786
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS413
Host range: Saccharomyces cerevisiae, Candida robusta; Escherichia coli

Features (with orientation and position when available):
marker(s): HIS3, →, 200-1395
terminator: CYC1, →, 2077-2337
MCS: SalI...XbaI, →, 2343-2401
promoter for expression: GPD, ←, 2401-3081
marker(s): ampR, ←, 4328-5258
replicon: CEN6/ARSH4, →, 5363-5876
Vector: p413 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS413
Marker(s): ampR, HIS3
Construct size (kb): 5.851
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
vector with low copy number
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.7; EcoRI--5.9; BamHI--5.9.
Low copy number shuttle expression vector.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

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Component of: ATCC 87669
Shipped frozen
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