p413 GPD (ATCC® 87354)

Applications: YX-type (expression) shuttle vectorvector with low copy number  /  Depositors: M Funk

Permits and Restrictions

View Permits

Designations p413 GPD
Depositors M Funk
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli HB101 (ATCC 33694)
Vector Information
Intact vector size: 5.786
Type of vector: plasmid
Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS413
Host range: Saccharomyces cerevisiae, Candida robusta; Escherichia coli

Features (with orientation and position when available):
marker(s): HIS3, →, 200-1395
terminator: CYC1, →, 2077-2337
MCS: SalI...XbaI, →, 2343-2401
promoter for expression: GPD, ←, 2401-3081
marker(s): ampR, ←, 4328-5258
replicon: CEN6/ARSH4, →, 5363-5876
Vector: p413 GPD (plasmid)
Promoters: Promoter for expression GPD
Construction: pRS413
Marker(s): ampR, HIS3
Construct size (kb): 5.851
Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GPD
replicon: CEN6/ARSH4
MCS: SalI...XbaI
terminator: CYC1
YX-type (expression) shuttle vector
vector with low copy number
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--5.2, 0.7; EcoRI--5.9; BamHI--5.9.
Low copy number shuttle expression vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C

Mumberg D, et al. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156: 119-122, 1995. PubMed: 7737504

Related Products
Component of: ATCC 87669
Shipped frozen
Product Sheet
Product Sheet
Product Sheet
Attachments Map
Vector sequence