YCpIF2 (ATCC® 87079)

Applications: YX-type (expression) shuttle vector  /  Depositors: RW Davis, PK Foreman

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Designations YCpIF2
Depositors RW Davis, PK Foreman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.5000000000000000
Vector: YCpIF2 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pUN105
Construct size (kb): 7.5
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SacI...SalI
centromere: CEN4
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): XbaI--7.5; BamHI--7.5; EcoRI--4.3, 3.5.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF1, YCpIF2 and YCpIF3 (ATCC 87078-87080) differ only in the reading frame of the multiple cloning site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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