YCpIF2 (ATCC® 87079)

Applications: YX-type (expression) shuttle vector  /  Depositors: RW Davis, PK Foreman

Designations YCpIF2
Depositors RW Davis, PK Foreman
Biosafety Level 1
Vector Information
Size (kb): 7.5000000000000000
Vector: YCpIF2 (plasmid)
Promoters: Promoter for expression GAL1
Construction: pUN105
Marker(s):LEU2,ampR
Construct size (kb): 7.5
Features: marker(s): LEU2
marker(s): ampR
promoter for expression: GAL1
replicon: ARS1
replicon: pMB1
MCS: SacI...SalI
centromere: CEN4
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XbaI--7.5; BamHI--7.5; EcoRI--4.3, 3.5.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF1, YCpIF2 and YCpIF3 (ATCC 87078-87080) differ only in the reading frame of the multiple cloning site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907

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component of:ATCC 87030
Shipped freeze-dried
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