pBG1 (ATCC® 87035)

Applications: contains sequence autonomous replication sequencecontains sequence imidazole acetol phosphate transaminaseshuttle vector  /  Depositors: KL Gould

Permits and Restrictions

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Designations pBG1
Depositors KL Gould
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.6999998092651370
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: cosmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Vector: pBG1 (plasmid)
Promoters: Promoter lac
Construction: pUC18, ars1, his3
Marker(s):ampR,his3
Construct size (kb): 5.699999809265137
Features: insert detection: lacZ'
marker(s): ampR
marker(s): his3+
promoter: lac
replicon: ars1
replicon: pMB1
MCS: SacI...SphI
Applications
contains sequence autonomous replication sequence
contains sequence imidazole acetol phosphate transaminase
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2; PstI--5.7.
The his3 insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.49; ClaI--0.04; EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27; XhoI--0.84.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burke JD, Gould KL. Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol. Gen. Genet. 242: 169-176, 1994. PubMed: 8159167

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component of:ATCC 87686
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