pBG1 (ATCC® 87035)

Applications: contains sequence autonomous replication sequencecontains sequence imidazole acetol phosphate transaminaseshuttle vector  /  Depositors: KL Gould

Permits and Restrictions

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Designations pBG1
Depositors KL Gould
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.6999998092651370
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: cosmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752
Vector: pBG1 (plasmid)
Promoters: Promoter lac
Construction: pUC18, ars1, his3
Marker(s):ampR,his3
Construct size (kb): 5.699999809265137
Features: insert detection: lacZ'
marker(s): ampR
marker(s): his3+
promoter: lac
replicon: ars1
replicon: pMB1
MCS: SacI...SphI
Applications
contains sequence autonomous replication sequence
contains sequence imidazole acetol phosphate transaminase
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2, 0.55; DraI/EcoRI--2.5, 2.4, 0.7; HindIII--3.5, 2.2; PstI--5.7.
The his3 insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.49; ClaI--0.04; EcoRI--0.47, 1.01; HindIII--1.03; PvuI--0.04; XbaI--0.27; XhoI--0.84.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Burke JD, Gould KL. Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol. Gen. Genet. 242: 169-176, 1994. PubMed: 8159167

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component of:ATCC 87686
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